Connectivity analyses are very much en vogue in fMRI (and EEG) studies. Here, we show one of our own examples applying state of the art dynamic functional connectivity analysis (focusing on EEG-defined awake epochsonly). This work was published as Laufs, H., R. Rodionov, R. Thornton, J. S. Duncan, L. Lemieux and E. Tagliazucchi (2014). "Altered FMRI connectivity dynamics in temporal lobe epilepsy might explain seizure semiology." Front Neurol 5: 175.
 

Temporal lobe epilepsy (TLE) can be conceptualized as a network disease. The network can be characterized by inter-regional functional connectivity, i.e., blood oxygen level-dependent (BOLD) signal correlations between any two regions. However, functional connectivity is not constant over time, thus computing correlation at a given time and then at some later time could give different results (non-stationarity).

BOLD signal variance in patients with temporal lobe epilepsy versus healthy control subjects

BOLD signal variance in patients with temporal lobe epilepsy in the hippocampi is higher than in controls (colorbar indicates p-value thresholded at p <0.001 fordisplay, clusters survive family-wise error correction at p <0.05).

We hypothesized (1) that non-stationarities can be induced by epilepsy (e.g., interictal epileptic activity) increasing local signal variance and that (2) these transient events contribute to fluctuations in connectivity leading to pathological functioning, i.e., TLE semiology. We analyzed fMRI data from 27 patients with TLE and 22 healthy controls. Testing hypothesis (1), we identified brain regions where the BOLD signal variance was significantly greater in TLE than in controls: the temporal pole - including the hippocampus. Taking the latter as the seed region and testing hypothesis (2), we calculated the time-varying inter-regional correlation values (dynamic functional connectivity) to other brain regions and found greater connectivity variance in the TLE than the control group mainly in the precuneus, the supplementary and sensorimotor, and the frontal cortices. We conclude that the highest BOLD signal variance in the hippocampi is highly suggestive of a specific epilepsy-related effect. The altered connectivity dynamics in TLE patients might help to explain the hallmark semiological features of dyscognitive seizures including impaired consciousness (precuneus, frontal cortex), sensory disturbance, and motor automatisms (sensorimotor cortices, supplementary motor cortex). Accounting for the non-stationarity and state-dependence of functional connectivity are a prerequisite in the search for potential connectivity-derived biomarkers in TLE.

ofsignificantly greatervarianceofhippocampaldynamicfunctionalconnectivityin patients withtemporallobeepilepsy(here:seedinlefthippocampus) than inhealthycontrols.

Significantly greater varianceof hippocampal dynamic functional connectivity in patients with temporal lobe epilepsy (here: seed in left hippocampus) than in healthy controls (colorbar indicates p-value thresholded at p <0.001 fordisplay, clusters survive family-wise error correction at p <0.05).

Arbeitsgruppen der Neurologie an der Christian-Albrechts-Universität zu Kiel

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